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2 609 641

(13)

(51)

IPC

C12N15/00(2006-01-01)

C07H21/04(2006-01-01)

(21) (22)

Application

2015152196, 2015-12-07

(24)

Start date

2015-12-07

(22)

Actual filing date

2015-12-07

(45)

Published

2017-02-02

(72)

Inventor

Ikonnikova Anna YurevnaNasedkina Tatyana VasilevnaZasedatelev Aleksandr Sergeevich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Nauki Institut Molekulyarnoj Biologii Im. V.A. Engelgardta Rossijskoj Akademii Nauk Ran)

METHOD OF ANALYSIS OF SOMATIC MUTATIONS IN THE BCR/ABL CHIMERIC GENE USING RT-PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) Russian patent published in 2017 - IPC C12N15/00 C07H21/04

Abstract RU 2609641 C1

FIELD: medicine.

SUBSTANCE: present invention relates to genetics, molecular biology and medicine. Method is presented by definition of somatic mutations in the tirozon-kinase domain of BCR / ABL gene, responsible for resistance to therapy with imatinib in chronic myeloid leukemia. Method is carried out using RT-PCR with subsequent hybridization with oligonucleotide biological microchip (biochip). Invention enables to determine 11 somatic mutations: M244V, G250E, Y253H, Y253F, E255K, E255V, T315I, M351T, F359C, F359I, F359V. Diagnosis of mutations allows to establish efficiency with a certain probability and appropriateness of treatment of patients with imatinib.

EFFECT: invention allows to perform analysis in a short time and with little material costs and can be used in medicine.

4 cl, 2 dwg, 2 tbl, 4 ex

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RU 2 609 641 C1

Authors

Ikonnikova Anna Yurevna

Nasedkina Tatyana Vasilevna

Zasedatelev Aleksandr Sergeevich

Dates

2017-02-02—Published

2015-12-07—Filed