METHOD OF ANALYSIS OF SOMATIC MUTATIONS IN THE BCR/ABL CHIMERIC GENE USING RT-PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) Russian patent published in 2017 - IPC C12N15/00 C07H21/04 

Abstract RU 2609641 C1

FIELD: medicine.

SUBSTANCE: present invention relates to genetics, molecular biology and medicine. Method is presented by definition of somatic mutations in the tirozon-kinase domain of BCR / ABL gene, responsible for resistance to therapy with imatinib in chronic myeloid leukemia. Method is carried out using RT-PCR with subsequent hybridization with oligonucleotide biological microchip (biochip). Invention enables to determine 11 somatic mutations: M244V, G250E, Y253H, Y253F, E255K, E255V, T315I, M351T, F359C, F359I, F359V. Diagnosis of mutations allows to establish efficiency with a certain probability and appropriateness of treatment of patients with imatinib.

EFFECT: invention allows to perform analysis in a short time and with little material costs and can be used in medicine.

4 cl, 2 dwg, 2 tbl, 4 ex

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RU 2 609 641 C1

Authors

Ikonnikova Anna Yurevna

Nasedkina Tatyana Vasilevna

Zasedatelev Aleksandr Sergeevich

Dates

2017-02-02Published

2015-12-07Filed