FIELD: biochemistry, molecular biology, in particular analytical systems based on DNA PCR-amplification.
SUBSTANCE: invention relates to composition for amplification reaction, comprising two heat-resistant enzymes, wherein one (major) component represents DNA polymerase, which is usually applied in PCR method, and the second (minor) component represents 3'-exonuclease from Archeoglobus fulgidus (Afu III exonuclease).
EFFECT: method for PCR-amplification with improved accuracy and increased validity of obtained test-system data.
10 cl, 13 dwg, 2 tbl, 13 ex
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