FIELD: biotechnology, genetic engineering, enzymes, biochemistry. SUBSTANCE: mutant alpha-isopropylmalate synthase (IPMS) is obtained by deletion, substitution, insertion or addition of one or some amino acid residues. Properties of mutant IPMS are identical with natural DNA encoding IPMS of wild type and comprises mutation that allows to loss sensitivity to inhibitory effect by L-leucine by feedback inhibition mechanism. DNA transforms Escherichia coli strain that is cultured for L-leucine preparing. Invention ensures to increase the yield of L-leucine that is used as food supplement, reagent and growth factor. EFFECT: improved method of strain and L-leucine preparing. 6 cl, 3 tbl
| Title |
Year |
Author |
Number |
| MUTANT N-ACETYLGLUTAMATE SYNTHASE (VARIANTS), DNA FRAGMENT, STRAIN OF MICROORGANISM ESCHERICHIA COLI AS PRODUCER OF ARGININE AND METHOD FOR PREPARING L-ARGININE |
2001 |
- Ptitsyn L.R.
- Al'Tman I.B.
- Smirnov S.V.
- Rostova Ju.G.
- Jampol'Skaja T.A.
- Leonova T.V.
- Gusjatiner M.M.
|
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| SMALL SUBUNIT OF ISOZYME III AND ISOZYME III OF ACETOHYDROXYACID SYNTHETASE FROM ESCHERICHIA COLI, DNA FRAGMENT (VARIANTS), STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF L-VALINE (VARIANTS) AND METHOD FOR PREPARING L-VALINE |
2000 |
- Livshits V.A.
- Doroshenko V.G.
- Gorshkova N.V.
- Belareva A.V.
- Ivanovskaja L.V.
- Khurges E.M.
- Akhverdjan V.Z.
- Gusjatiner M.M.
- Kozlov Ju.I.
|
RU2209246C2 |
| MUTANT PROTEIN, CODED BY GENE yddG, AND METHOD OF OBTAINING AROMATIC L-AMINO ACIDS WITH APPLICATION OF BACTERIA OF GENUS Escherichia |
2012 |
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- Tsyrenzhapova Irina Sergeevna
|
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| METHOD FOR PREPARING NORLEUCINE AND NORVALINE USING MICROORGANISM OF Escherichia GENUS WHEREIN ALL ACETOHYDROXYACID SYNTHASES ARE INACTIVATED |
2004 |
- Stojnova Natalija Viktorovna
- Sycheva Elena Viktorovna
- Preobrazhenskaja Ekaterina Sergeevna
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- Matrosov Nikolaj Georgievich
|
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2012 |
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- Ptitsyn Leonid Romanovich
|
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2002 |
- Gusjatiner M.M.
- Voroshilova Eh.B.
- Rostova Ju.G.
- Ivanovskaja L.V.
- Lunts M.G.
- Khurges E.M.
|
RU2243260C2 |
| METHOD OF L-THREONINE RECEIVING WITH USAGE OF BACTERIA BELONGING TO GENUS Escherichia, IN WHICH INACTIVATED GENE lrhA |
2006 |
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|
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| METHOD FOR PREPARING L AMINO ACIDS, STRAIN ESCHERICHIA COLI AS PRODUCER OF L-AMINO ACID (VARIANTS) |
2001 |
- Tabolina E.A.
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|
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| L-THREONINE TECHNIQUE USING BACTERIUM OF Escherichia GENUS WITH INACTIVATED yrbG GENE |
2006 |
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|
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2006 |
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|
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