METHOD FOR ENHANCEMENT OF PROLIFERATIVE ACTIVITY OF MARINE INVERTEBRATES Russian patent published in 2003 - IPC

FIELD: biotechnology. SUBSTANCE: invention relates to methods for enhancement of proliferative activity of cells of invertebrate animals, namely, to urchin embryonic cells. Proposed method involves administration in urchin embryonic cells prepared after dissociation of blastulas the foreign gene ga14 as component of plasmid pMA 563. Then transgene cells are treated with 5-10% solution of polyethylene glycol of molecular mass 3000-4000 Da. Proposed method ensures to enhance proliferative activity of urchin cells significantly and to prepare cells with the high growth potential. Invention can be used in cellular biology and marine technology for preparing constant cellular cultures of marine invertebrates followed by their usage in scientific and practice aims and for preparing biologically active substances. EFFECT: improved effectiveness of method. 2 cl, 5 dwg, 3 ex