METHOD OF OBTAINING BOTULINUM NEUROTOXIN (VERSIONS) Russian patent published in 2015 - IPC C07K1/18 C07K14/33 B01D15/36 C07K1/20 A61K39/08 

Abstract RU 2561459 C2

FIELD: chemistry.

SUBSTANCE: group of inventions relate to field of biotechnology. Claimed are methods of obtaining biologically active botulinum neurotoxin with application of chromatography, in fact without application of animal-derived products. Cultivation of bacteria Clostridium botulinum in culture medium in fact without application of animal-derived products is realised. After that, bacteria are fermented into fermentation medium in fact without application of animal-derived products. At least one culture medium and fermentation medium includes protein of vegetable origin. Fermentation medium is collected by removal of cell residues. Collected medium is concentrated by filtration. Concentrated medium is diluted with buffer. Anion-exchange chromatography is carried out. Botulinum neurotoxin is eluted from anion-exchange medium with obtaining first eluent. First eluent is passed through cation-exchange medium with obtaining second eluent. In one version of method second eluent is processed with diafiltration. After that, processed eluent is filtered with obtaining complex of biologically active botulinum neurotoxin of A type, possessing activity 2.0×107 - 6.0×107 units/mg. In other version of method second eluent is filtered with obtaining biologically active botulinum neurotoxin, containing 1 ng or less of residual nucleic acid per each mg of obtained botulinum neurotoxin, with realising method within one week or less.

EFFECT: inventions provide fast and effective purification of botulinum neurotoxin within short time period, homogeneity of product characteristics from batch to batch.

8 cl, 1 dwg, 3 ex

Similar patents RU2561459C2

Title Year Author Number
METHOD FOR PURIFYING OF BOTULINUM TOXIN 2020
  • Kim, Dzhe Eun
  • Nam, Dzhon Sun
  • Kim, Seunkho
  • Chkhoi, Seun Kvan
  • Yun, Bum Dzhin
  • Chkhoi, Dzhin Khee
RU2805226C2
METHOD FOR PREPARING PURIFIED PREPARATION OF BOTULINIC TOXIN TYPE A 2002
  • Grachev O.E.
  • Elagin G.D.
  • Dubrovin M.Ju.
  • Bakulina L.V.
  • Rudoj B.A.
  • Pjatkov V.A.
  • Telitsyn L.M.
RU2230325C2
METHOD FOR PURIFICATION OF BOTULINUM TOXIN 2020
  • Kim, Dzhe Eun
  • Nam, Dzhon Sun
  • Kim, Seunkho
  • Chkhoi, Seun Kvan
  • Lim, Khyun Tek
  • Chkhoi, Dzhin Khee
RU2795197C1
METHOD OF PRODUCING BOTULINUM TOXIN 2016
  • Kim Kyoung-Yun
  • Kim Chung Sei
  • Kim Myung Seob
  • Sul Hye-Young
RU2707255C1
METHOD FOR BOTULINUM TOXIN PRODUCTION 2014
  • Kim Chon Sej
  • Son Kvan En
  • Min Ken Min
  • An En Tuk
RU2627159C2
METHOD FOR PRODUCTION OF NEUROMUSCULAR RELAXANT DRUG FOR TREATMENT OF MUSCLE DYSTONIAS 2005
  • Zajnullin Rustehm Rashatovich
  • Mel'Nikov Nikolaj Vladimirovich
  • Zagidullin Nail' Vilenovich
  • Alsynbaev Makhamat Makhamatullovich
  • Khaziev Artur Fatykhovich
  • Nigamov Farit Nigamovich
RU2292910C2
DEVICES AND METHOD TO PRODUCE HIGHLY CLEANED NEUROTOXINS 2010
  • Pfajl' Mikhaehl'
  • Fridrikh Jozef
  • Tehjlor Kharol'D V.
  • Ajzele Karl-Khajnts
  • Brjunn Kornelija
RU2571212C2
STRAIN OF HYBRID ANIMAL CELLS Mus musculus 1G7 - PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC FOR BOTULINUM TOXIN OF TYPE B 2014
  • Ivashchenko Tat'Jana Aleksandrovna
  • Belova Elena Valentinovna
  • Mitsevich Irina Petrovna
  • Mitsevich Evgenij Viktorovich
  • Kozyr' Arina Vladimirovna
  • Kolesnikov Aleksandr Vladimirovich
  • Djatlov Ivan Alekseevich
  • Shemjakin Igor' Georgievich
RU2571208C1
MEDICINAL PREPARATION FOR TREATMENT OF MUSCLE DYSTONIA AND METHOD FOR ITS PREPARING 2002
  • Vertiev Ju.V.
RU2206337C1
METHOD OF PRODUCING PROTEOLYTICALLY PROCESSED POLYPEPTIDE 2012
  • Rummel Andreas
RU2673910C2

RU 2 561 459 C2

Authors

Ton Dzhennifer L.

Patel Khehmant A.

Bates Ronal'D K.

Akhmad Vajdi M.

Dates

2015-08-27Published

2010-07-12Filed