METHOD FOR CULTURING MYCOBACTERIUM TUBERCULOSIS MICROORGANISMS Russian patent published in 2003 - IPC

Abstract RU 2209829 C2

FIELD: microbiology, medicine, biotechnology. SUBSTANCE: method involves addition of Mycobacterium tuberculosis to liquid nutrient medium and culturing in indicated nutrient medium. Liquid nutrient medium for mammalian cell cultures with monolayer of these cell cultures on backing taken in the concentration 10⁵cells/cm², not less, is used as nutrient medium and culturing Mycobacterium tuberculosis is performed in common with mammalian cell culture to the detachment stage of latter from backing. Liquid nutrient medium for mammalian cell cultures comprises total protein in the concentration 60 g/l, not less. Transplantable strain of cells VERO is used as mammalian cell culture. Medium BSK-II is used as liquid nutrient medium for mammalian cell cultures. Culturing method is approached to the natural conditions that provide possibility of prolonged culturing without loss of sensitivity against antituberculosis preparations and without alternations of biological properties. EFFECT: improved culturing method. 4 cl, 7 ex

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RU 2 209 829 C2

Authors

Durymanov A.G.

Rassadkin Ju.N.

Alekseev A.Ju.

Shestapalov A.M.

Repin V.E.

Dates

2003-08-10—Published

2001-08-20—Filed