IMMUNOGENIC COMPOSITION CONTAINING CHIMERIC HIV POLYPEPTIDE, POLYPEPTIDES AND TEST-SYSTEM FOR DETECTION OF ANTIBODIES RAISED TO HIV Russian patent published in 2003 - IPC

FIELD: medicine, biotechnology. SUBSTANCE: invention relates to immunogenic composition containing chimeric HIV polypeptide, method for induction of immune response to HIV 1 and HIV 1/2, chimeric polypeptide HIV 1 and HIV 1/2, DNA molecule encoding chimeric polypeptide, plasmid vector for expression of chimeric polypeptide, polyclonal antibodies raised to HIV 1 or HIV 1/2 and diagnostic test-system for detection of antibodies raised to HIV 1 and HIV 2. Invention involves immunogenic composition based on chimeric polypeptides HIV 1 or HIV 1/2 representing full-sized protein p24 fused with immunodominant fragment gp 41 of site 575-616 or taken among them (rec 24-41) or fused with immunodominant fragment gp 36 of the same site or taken among them (rec 24-36). Indicated composition is able to induce the strong immune response to HIV 1 or HIV 2. Invention involves also the gene construction able to expression of indicated chimeric polypeptides in E. coli cells and diagnostic test-system for detection of antibodies raised to HIV 1 and HIV 2. The advantage of invention involves the development of gene construction allowing expression the chimeric recombinant protein that contains a full-sized polypeptide as a component of a single polypeptide chain and related by its conformation to viral protein p24 and immunogenic protein fragment of HIV 1 or HIV 2 envelope. Indicated recombinant proteins are able to induce strong immune response to HIV 1 or HIV 1/2 that allows using these chimeric polypeptides both for creation of diagnostic test-systems and for their recommendation for development of vaccine preparations. EFFECT: valuable properties of composition. 18 cl, 3 tbl, 5 dwg, 10 ex