FIELD: medicine, immunology. SUBSTANCE: the present innovation deals with detecting the quantity of lymphocytes activated with an antigen in the culture of mononuclear cells of peripheral blood due to cytofluorimetric method by applying monoclonal antibodies to lymphocytes and at the difference of CD69+ lymphocytes being 4% and more during the first day or CD25+ lymphocytes 6% and more on the third day against negative control after introduction of antigen into patient's blood sample one should diagnose allergic reaction. The method provides broadened functional possibilities and availability and shortened terms of studying. EFFECT: higher efficiency of diagnostics. 2 tbl
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