FIELD: biotechnology, microbiology, amino acids. SUBSTANCE: L-amino acids of L- glutamic acid family are prepared by culturing Escherichia coli in nutrient medium. After accumulation L- amino acids are isolated from cultural fluid. The strain with enhanced capacity to produce L-amino acids of L- glutamic acid family that is caused by enhancement activity of protein encoded by gene b3434 is used as the strain-producer. The strain Escherichia coli 328 (pYHGN) is used as producer of L-arginine and the strain Escherichia coli 702ilvA (pYHGN) as producer of L-proline. Invention provides preparing L-amino acids with high degree of effectiveness. EFFECT: improved preparing method. 9 cl, 4 tbl, 1 dwg, 4 ex
Authors
Dates
2003-11-10—Published
2001-06-28—Filed