METHOD OF MULTIPLE PARALLEL SCREENING OF BINDING SPECIFICITY OF BIOLOGICALLY ACTIVE COMPOUNDS WITH NUCLEIC ACIDS USING BIOCHIP (VERSIONS) Russian patent published in 2002 - IPC

FIELD: molecular biology, medicine, pharmacology, environment protection. SUBSTANCE: biochip with immobilized oligonucleotides is prepared and hybridization of these nucleotides with a mixture of nonself-complementary oligonucleotides labeled with fluorescent label is carried out. Double-stranded oligonucleotides are formed on biochip that's are subjected for melting recording data and biochip is washed out. The repeated hybridization is carried out with the same mixture of oligonucleotides labeled with fluorescent label followed by incubation of biochip with the compound to be studied. Double-stranded oligonucleotides are melted again on biochip being these nucleotides are in complex with biologically active compound to be studied. Data are recorded and melting points of double-stranded oligonucleotides are determined in the presence and absence of compound to be studied and difference of melting point is measured. Based on total data obtained the specificity of binding of compound to be studied is determined. The universal biochip where in its units all possible hexanucleotides are immobilized is used preferably. Fluorescent dye can be Texas red (Texas Red). Oligonucleotides are melted using a thermotable. The mass of experimental data is treated using the computer program preferably. Dye Hoechst 33258 or protein HU can be used as compound to be studied. By the second variant method involves incubation of biochip with fluorescent compound to be studied immediately after preparing biochip with oligonucleotides immobilized on its. Method ensures to carry out the comparative experimental analysis of relationship degree of chemical compound to all possible sequences of nucleic acid in the range of binding site. EFFECT: improved method of screening. 20 cl, 8 dwg, 3 ex