METHOD FOR DETECTING VIRAL CONCENTRATION IN LIQUID BIOLOGICAL MATERIAL AND DEVICE FOR ITS IMPLEMENTATION Russian patent published in 2004 - IPC

FIELD: medicine, biotechnology, virology. SUBSTANCE: the present innovation deals with sampling tested material in liquid form followed by diluting the samples at preset "k" coefficient. Further sampling of test cells being of high specificity to tested viral type, and detection of average viral quantity "β" providing infection of the cells mentioned. Further dilution of samples with test cell suspension in accounting cell at preset cell concentration "C_c" should be performed. Then comes the next detection of viral concentration "C_v" due to transferring the mixture of tested material samples at preset cell concentration "C_c" into measuring cuvette. Moreover, one should form heterogeneous alternating electric field to measure average movement rate of every cell in suspension. Then one should determine the portion of immobile cells by the following formula: α = A/B followed by detecting viral concentration by the following formula: C_v = C_c•α•β•к. It is also suggested to apply a device for detecting viral concentration. This device has transparent measuring cuvette where electrodes are located connected with power supply source, and a measuring block, as well. Power supply source is a generator of alternating electric tension. Measuring block contains a microscope optically connected with a measuring cuvette, except this it has a system for picture analysis including video camera optically connected with a microscope, and a computer connected with video camera. The suggested method and device enable to detect viral concentration during shorter period of time, decrease labor capacity of the process and automatize measuring process. Innovations could be applied for detecting viral concentration in suspensions and, also, in industrial production to control the purity of technological liquids. EFFECT: higher efficiency of detection. 4 cl, 2 dwg, 1 tbl