METHOD OF DETERMINING COMPLEX PERMITTIVITY OF A BIOLOGICAL CELL IN SUSPENSION Russian patent published in 2019 - IPC G01N33/487 

FIELD: medicine.

SUBSTANCE: invention refers to medicine and can be used in determining complex dielectric permeability of biological particles and cells. For this purpose, a suspension of biological cells in 0.3 M sucrose solution is placed between electrodes of a measuring cell and an inhomogeneous alternating electric field (IAEF) is created. At the stage of interaction of the biological cell with the IAEF, searching for the equilibrium frequency ω_e, on which the translational movement of the biological cell in suspension relative to the electrodes of the measuring cell and its rotational movement about its own axis is completely stopped. When field frequency is changed, said equilibrium frequency ω_e is measured using a frequency meter. Specific resistance of biological cells suspension ρ_cp are determined by bridge method at frequency ω_e. Biological cell radius r_cell and relative dielectric permeability ε_cp cell suspension is calculated through equation of capacity of flat capacitor. Complex dielectric permeability ε*_cell cells are calculated as square root of sum of squares of relative dielectric permeability of cell suspension ε_cp and its imaginary part ε'_cp at equilibrium frequency ω_e.

EFFECT: invention provides determining the dielectric permittivity of a living biological cell.

1 cl, 1 tbl, 2 dwg, 1 ex