FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, environmental and industrial toxicology. Disclosed is method of determining cytotoxicity of nano materials based on zinc oxide. Nano material is prepared in form of two-layer nano structure, where upper layer is modified by Fe atoms. Obtained nano structure is applied on dielectric substrate, to which current-conducting contact platforms are formed connected by contact conductors with measuring device. Substrate, nano material, contact pads and contact conductors form biosensor. To determine cytotoxicity measure time dependence of sensor response is change in resistance, capacitance or EMF film at interaction of its surface with bacterial suspension. Sensor response of two-layer nano structure ZnO-ZnO:Fe is proportional to concentration of living bacteria. On basis of obtained relationship time during which concentration of bacteria decreases in e times due to their death on film surface is determined.
EFFECT: technical result is wider range of analysed materials.
1 cl, 4 dwg, 1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF NANOCARBON ANALYSIS FOR BIOTOXICITY | 2010 |
|
RU2437938C2 |
| METHOD OF PRODUCING GAS SENSOR BASED ON THERMOVOLTAIC EFFECT IN ZINC OXIDE | 2015 |
|
RU2613488C1 |
| METHOD OF MAKING GAS SENSOR BASED ON MECHANICALLY ACTIVATED POWDER OF ZINC OXIDE AND GAS SENSOR BASED ON IT | 2019 |
|
RU2718710C1 |
| METHOD FOR DETECTION OF COPPER IONS IN THE ENVIRONMENT AND BIOSENSOR FOR ITS IMPLEMENTATION | 2015 |
|
RU2628704C2 |
| USE OF 1,3-DIHYDROXYBENZOL AS SENSITIZERS OF BACTERIAL CELLS TO DAMAGING ACTION OF NANO-STRUCTURED CARBON COMPOUNDS | 2014 |
|
RU2598731C2 |
| METHOD OF PRODUCING A GAS SENSOR WITH A NANOSTRUCTURE WITH A SUPER-DEVELOPED SURFACE AND A GAS SENSOR BASED THEREON | 2018 |
|
RU2687869C1 |
| CHEMORESISTIVE GAS SENSOR AND METHOD FOR ITS MANUFACTURE | 2023 |
|
RU2806670C1 |
| METHOD OF PRODUCING THIN LAYERS OF GRAPHENE OXIDE WITH FORMATION OF A SUBLAYER OF CARBON NANOTUBES | 2018 |
|
RU2693733C1 |
| USE OF GRAPHENE OXIDE TO ACTIVATE PHAGOCYTIC FUNCTION OF MACROPHAGES | 2022 |
|
RU2809372C1 |
| METHOD FOR OBTAINING NANOLITHOGRAPHY DRAWINGS WITH AN ORDERED STRUCTURE WITH AN OVER-DEVELOPED SURFACE | 2021 |
|
RU2757323C1 |
