FIELD: microbiology, ecology. SUBSTANCE: invention relates to methods for modeling symbiosis (biocenosis) of meliodosis pathogen with saprophyte slipping microorganisms and the following detection of cells B. pseudomallei in this symbiosis. Method involves seeding cultures B. pseudomallei and M. xanthus by streak method on surface of solid nutrient medium in the definite picture. After additional culturing seeding at 34 C for 72-96 h cells of meliodosis pathogen are included into fold-pockets of M. xanthus fruit bodies. Part of B. pseudomallei cells are lysed inside of fruit bodies but some cells of meliodosis pathogen are adapted to the new habitation medium, persisted in it and propagated even so, i. e. cells show symbiosis. For detection of B. pseudomallei cells in fruit bodies of M. xanthus biomass of fruit bodies containing single cells of meliodosis pathogen presumably is cultured additionally preliminary in nutrient broth, treated with chloroform and chloroform lysate is used as donor material (i. e. DNA preparation) for carrying out the genetic transformation. Genetic transformation is carried out in minimal medium by auxotrophy marker using the univercal recipient P. pseudomallei SVBK 141 Pur. The presence of cells B. pseudomallei in fruit bodies is estimated indirectly by formation of prototrophic colonies (recombinants) in minimal medium that appear as result of genetic transformation purine-dependent cells of universal recipient with donor material. Invention provides the development of biological model of symbiosis of pathogenic microorganism (B. pseudomallei) with ubiquist wide-spread saprophyte (M. xanthus), allows to detect single cells of meliodosis pathogen in biomass of this biocenosis that can be used for investigation of ecological systems and assay of ecological valence of components. EFFECT: improved modeling method. 2 cl, 1 dwg, 3 ex
