FIELD: medicine, infectious diseases.
SUBSTANCE: invention relates to a method for preparing capsule antigen of meliodosis pathogen eliciting anti-phagocytic activity. Method involves culturing the strain B. pseudomallei, inactivation, isolation of glycoprotein by gel-filtration and ion-exchange chromatography methods resulting to preparing glycoprotein with molecular mass 200 kDA consisting of 90% of carbohydrates, 10% of proteins and comprising 2.17% of nitrogen, 46.4% of carbon and 8.09% of hydrogen. Advantage of method involves preparing the purified glycoprotein eliciting anti-phagocytic activity.
EFFECT: improved method for preparing, valuable properties of antigen.
2 dwg, 5 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR DETERMINING BURKHOLDERIA MALLEI CAPSULAR ANTIGEN IMMUNOGENICITY LEVEL | 2005 |
|
RU2293993C1 |
METHOD FOR DIFFERENTIATION PATHOGENIC AND NONPATHOGENIC BURKHOLDERIA | 2008 |
|
RU2378360C1 |
METHOD FOR PREPARING MELIODOSIS PATHOGEN CAPSULE SUBSTANCE | 2001 |
|
RU2231364C2 |
METHOD FOR DIFFERENTIATION OF MALLEUS AND MELIOIDOSIS EXITANTS WITH PRECIPITATING ANTISERUM | 2006 |
|
RU2305557C1 |
METHOD OF MELIOIDOZE ANTIGEN ESTIMATE FOR PROTECTIVITY LEVEL | 2007 |
|
RU2354400C1 |
STRAIN OF CULTIVATED HYBRID ANIMAL CELLS MUS MUSCULUS L BPM VD-10 - PRODUCER OF MONOCLONAL ANTIBODY 5H/E TO ANTIGEN KDA 200 OF MELIOIDOSIS PATHOGEN | 2014 |
|
RU2570638C1 |
METHOD FOR DETECTING CROSS-REACTING ANTIGENS OF THE AGENTS OF MELIOIDOSIS, GLANDERS, PLAGUE, TULAREMIA AND TUBERCULOSIS | 2004 |
|
RU2286576C2 |
METHOD FOR IMMUNOPROPHYLAXIS OF EXPERIMENTAL PSEUDOCHOLERA WITH ENTRAPPED ANTIGENS Burkholderia pseudomallei | 2008 |
|
RU2373955C1 |
STRAIN OF CULTIVATED HYBRID ANIMAL CELLS MUS MUSCULUS L BPM VD-11 - PRODUCERS OF MONOCLONAL ANTIBODY 6A/G TO ANTIGEN 200 kDA OF MELIOIDOSIS PATHOGEN | 2014 |
|
RU2570634C1 |
STRAIN OF BACTERIUM BURKHOLDERIA THAILANDENSIS KM 161 AS AVIRULENT MELIODOSIS PATHOGEN SIMULATOR | 2004 |
|
RU2257413C1 |
Authors
Dates
2005-07-10—Published
2003-06-30—Filed