VECTOR FOR EXPRESSION AND SECRETION OF HUMAN ALPHA-INTERFERON (HIFN(hIFNα)), STR AIN ESCHERICHIA COLI FOR EXPRESSION AND SECRETION OF HIFNhIFNα, (VARIANTS) AND METHOD FOR PRODUCING HIFNhIFNα, Russian patent published in 2004 - IPC

FIELD: biotechnology, protein engineering, molecular biology. SUBSTANCE: invention relates to producing recombinant human interferons. Expressing vector for secretory producing human alpha-interferon (hIFNhIFNα) comprises polynucleotide encoding the modified signal sequence of thermostable E. coli enterotoxin II and polynucleotide encoding hINFhIFNα ligated with its 3-end. Vector is used in method for secretory producing human interferon by transformation the strain E. coli and culturing the indicated strain. Method provides secreting the soluble form of active hIFNhIFNα no comprising the additional methionine residue at its N-end into E. coli cell periplasm. EFFECT: valuable properties of vector and strain. 13 cl, 6 dwg, 1 tbl, 5 ex