MODIFIED SIGNAL PEPTIDE OF ESCHERICHIA COLI ENTEROTOXIN-II AND MICROORGANISM EXPRESSING FUSION PROTEIN OF MENTIONED PEPTIDE WITH HETEROLOGICAL PROTEIN Russian patent published in 2003 - IPC

FIELD: biochemistry, peptides. SUBSTANCE: invention relates to the modified signal peptide of E. coli enterotoxin-II characterized by that at least one of amino acids in positions, 2, 4, 5, 12, 20 and 22 of signal peptide of E. coli enterotoxin-II with amino acid sequence Met-Lys-Lys-Asn-Ile-Ala-Phe-Leu-Leu-Ala- -Ser-Met-Phe-Val-Phe-Ser-Ile-Ala-Thr-Asn-Ala-Tyr-Ala is replaced for another amino acid under condition that at least one of amino acids in positions 2 and 4 of the modified peptide is lysine. Invention describes also gene encoding indicated peptide and plasmid vector comprising this gene also. Invention describes strains of E. coli transformed with plasmid vector and used for human growth hormone preparing and method of human growth hormone preparing in microorganisms. EFFECT: improved method of peptide preparing. 12 cl, 3 tbl, 5 dwg, 12 ex