HEAT-INDUCED PROMOTER AND ITS USING Russian patent published in 2004 - IPC

FIELD: biotechnology, molecular biology, biochemistry.

SUBSTANCE: invention relates to heat-induced promoters, sets and methods for preparing one or more amount of proteins using heat-induced promoter. Heat-induced promoter is taken among the following nucleic acids: (a) nucleic acid with sequence indicated in the description; (b) nucleic acid with sequence that elicits at least 40% of identity in the site with length of 300 nucleotide pairs and with sequence indicated in (a); (c) nucleic acid that is hybridized with complementary chain of one of nucleic acid under rigid conditions and indicated in (a) or (b); (d) fragment of one of nucleic acid indicated in (a) - (c) that retains function of heat-induced promoter; (e) combination of some nucleic acids indicated in (a) - (d) wherein sequences of nucleic acids can be differ or to be similar; or nucleic acid having sequence that is complementary to one of nucleic acids indicated in (a) - (e). Invention represents vector used for expression of proteins and carrying heat-induced promoter. Vectors is a component of set for expression of proteins in common with host-cell suitable for induction of heat-induced promoter and for producing recombinant protein. Invention provides preparing promoter with the selective index of heat induction, as it is possible, in particular, promoter that is active in yeast and suitable for expression of protein at high temperature.

EFFECT: valuable biological properties of promoter.

32 cl, 16 dwg, 1 tbl, 5 ex