METHOD OF PRODUCING HYALURONAN IN RECOMBINANT HOST CELL Russian patent published in 2009 - IPC C12P19/04 C12N15/52 C12N15/62 C12N1/21 

FIELD: chemistry; biochemistry.

SUBSTANCE: present invention pertains to biotechnology and can be used in biomedicine for producing hyaluronan. Proposal is given of a method of producing hyaluronan, involving culturing Bacillus host cells in conditions, suitable for obtaining hyaluronan, and extraction of the target product from the culture medium. The Bacillus host cell contains a genetic structure, comprising a promoter, functionally active in the given cell, and encoding a region, consisting of a nucleotide sequence, encoding streptococcal hyaluronansynthase (hasA); sequence encoding UDP-glucose-6-dehydrogenase Bacillus (tuaD) or a similar enzyme of streptococcal origin (hasB), and a sequence, encoding bacterial or streptococcal UDP-glucose pyrophosphorylase (gtaB and hasC respectively). Use of the invented method provides for production of considerable quantities of hyaluronan with good examined, nonpathogenic cellular system.

EFFECT: obtaining considerable of hyaluronan with good examined, nonpathogenic cellular system.

15 cl, 45 dwg, 2 tbl, 20 ex