FIELD: genetic engineering, molecular biology, medical-biological industry.
SUBSTANCE: by screening mouse cDNA library the nucleotide sequence encoding protein thrombopoietin is isolated. This protein elicits ability to stimulate MPL-dependent proliferation or differentiation of cell-precursors of myeloid or lymphoid order. Vectors for expression of the prepared encoding sequence in yeast and mammalian cells are constructed. Culturing eucaryotic host-cells transformed with proposed expressing vectors provides preparing significant amount of recombinant thrombopoietin.
EFFECT: improved preparing method.
13 cl, 2 dwg, 5 tbl, 15 ex
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