SELECTIVE AGONISTS AND ANTAGONISTS OF IL-2 Russian patent published in 2004 - IPC

FIELD: molecular biology, proteins, biochemistry, pharmacy.

SUBSTANCE: invention relates to polypeptide (I) representing the mutant protein of human IL-2 (interleukin-2) wherein human IL-2 is substituted by at least one position (20, 88 or 126) that results to ability of mutant protein to activate T cells as compared with natural killer cell preferably. Invention relates also to a pharmaceutical composition eliciting with immunomodulating activity and comprising polypeptide (I); polynucleotide with DNA sequence encoding human mutant protein IL-2; vector pBC1IL 2SA used for expression of human mutant protein IL-2; Chinese hamster ovary cell line; African green grivet cell line; strain E. coli; Spodoptera fugiperda cell strain transformed with vector pBC1IL 2SA that produce the human mutant protein IL-2; method for treatment of mammals suffering with oncological diseases and to a method for selection of mutant proteins IL-2 based on their estimation in investigations using IL-2Rαβγ in comparison with IL-2Rβγ wherein activity of mutant protein IL-2 is enhanced with respect to IL-2 of wild type in some investigation as compared with another one.

EFFECT: valuable medicinal properties of polypeptide.

26 cl, 8 tbl, 20 dwg, 11 ex