ISOLATED POLYNUCLEOTIDE ABLE TO CONFER TO PLANT RESISTANCE OR TOLERANCE TO GLYFOSATE HERBICIDE, VECTOR, METHOD FOR PREPARING PLANTS WITH TOLERANCE OR RESISTANCE TO GLYFOSATE HERBICIDE, METHOD FOR REGENERATION OF TRANSFORMED PLANT AND METHOD FOR SELECTIVE CONTROL OF WEEDS Russian patent published in 2004 - IPC

FIELD: biotechnology, molecular biology, herbicides, agriculture.

SUBSTANCE: invention relates to technology of recombinant DNAs that are required for preparing transgenic plants with resistance or eliciting significant tolerance to herbicides. Method involves isolation of polynucleotide encoding chloroplast signal peptide and glyfosate-resistant rice 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) located by course of transcription after site encoding chloroplast signal peptide. Expression of indicated site is under control of vegetable functional promoter that is not heterologous with respect to indicated site and chloroplast signal promoter is not heterologous with respect to indicated synthase. Indicated components are in sequence by direction 5' 3' of transcription with possibility of variants in disposition of enhancers. Taken together, the sequence encoding rice EPSPS is modified by such manner that by the first position in conservative site GNAGTAMRPLTAAV in enzyme of wild type residue Thr is replaced for residue Ile and by the second position residue Pro is replaced for Ser and thus the modified sequence represents GNAGIAMRSLTAAV. Indicated polynucleotide is inserted in vector construction that is used for transformation of vegetable material. Prepared transformed material is subjected for preparing whole plants with resistance or tolerance to glyfosate herbicide. Regeneration involves preparing plant tissue that will be transformed, placing tissue transformed with above indicated polynucleotide or vector in nutrient medium containing compound providing possibilities for identification or selection of transformed tissues taken for regeneration, transfer of formed, at least one, sprout in the second medium promoting to formation of roots and culturing indicated sprout up to stage of adult plant that is able to multiplication. Field with agriculture transgenic plants and contaminated with weeds is treated with glyfosate taken in effective dose to control weeds and without significant effect on agricultural plants.

EFFECT: reduced industrial consumptions, enhanced yield of agricultural production.

14 cl, 17 dwg, 2 tbl, 20 ex