METHOD FOR DIAGNOSING OXIDATION STRESS IN HUMAN ORGANISM Russian patent published in 2004 - IPC

FIELD: medicine.

SUBSTANCE: method involves determining thiol groups concentration in hemolysate. Disulfide groups quantity is determined by calculating difference between thiol groups quantity in practically healthy people hemolysate equal to 0.174±0.004 optical units and one of person under study. The difference being equal to 0.000±0.008 optical units, no oxidation process taking place is to be determined. The difference being positive, quantity of intermediate and minor products of molecule oxidation biomolecules like proteins, lipids, hydrocarbons, and nucleins entering in reaction with thiobarbituric acid is additionally determined. Biomolecules products quantity is additionally determined after carrying out preliminary chemical induction of peroxidation processes with Fe²⁺. Erythrocyte biomolecule oxidation modification coefficient is determined from formula BOMCer =(TBNer+TBNerInd)(E_d-sh-gr - E_i-sh-gr)100, where BOMCer is the erythrocyte biomolecule oxidation modification coefficient in oxidation activity units OAU, TBNer is the quantity of intermediate and minor products of molecule oxidation biomolecules like proteins, lipids, hydrocarbons, and nucleins entering in reaction with thiobarbituric acid in optical units, TBNerInd is the quantity of intermediate and minor products of molecule oxidation biomolecules like proteins, lipids, hydrocarbons, and nucleins entering in reaction with thiobarbituric acid induced with Fe²⁺ in optical units, E_d-sh-gr is the number of hemolysate thiol groups in practically healthy people groups in optical units, E_i-sh-gr is the number of hemolysate thiol groups in patients under examination in optical units, 100 is the calculated calculation coefficient. The higher is the BOMCer value, the higher is organism oxidation stress level.

EFFECT: high accuracy of diagnosis.