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2 236 454

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IPC

(21) (22)

Application

2002111436/13, 2002-04-30

(24)

Start date

2002-04-30

(22)

Actual filing date

2002-04-30

(45)

Published

2004-09-20

(72)

Inventor

Tarakanov B.V.Aleshin V.V.Karpushina S.G.

(73)

Holder

Tarakanov Boris Vasil'EvichAleshin Vladimir VeniaminovichKarpushina Svetlana Grigor'EvnaGosudarstvennoe Nauchnoe Uchrezhdenie Vserossijskij Nauchno-Issledovatel'Skij Institut Fiziologii, Biokhimii I Pitanija Sel'Skokhozjajstvennykh Zhivotnykh

METHOD FOR CONSTRUCTION OF RECOMBINANT STRAINS OF MICROORGANISMS EXPRESSING PROCARYOTIC AND EUCARYOTIC GENES Russian patent published in 2004 - IPC

Abstract RU 2236454 C2

FIELD: biotechnology, genetic engineering, microbiology.

SUBSTANCE: invention relates to a method for construction of recombinant strains of microorganisms that provides expression of the target prokaryotic or eucaryotic gene in any strain of microorganism maintaining replication of vector DNA. Based on the multicopy plasmid pLF1311 a vector is constructed providing carrying out easy selection of cloned sequences by phenotype trait of microorganisms carrying it and to express their. Using this vector the probiotic starin Lactobacillus sp. BPA3 (pLF-SL2) is constructed that expresses eucaryotic gene encoding somatoliberin. This invention is used for the development of probiotics and ferments - preparations based on live cultures of microorganisms.

EFFECT: improved method for construction.

1 dwg, 2 ex

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RU 2 236 454 C2

Authors

Tarakanov B.V.

Aleshin V.V.

Karpushina S.G.

Dates

2004-09-20—Published

2002-04-30—Filed