PROCESS AND LINE FOR PRODUCTION OF BIOLOGICAL PREPARATIONS BASED ON ENTOMONOPATHOGENIC NEMATODES Russian patent published in 2004 - IPC

FIELD: microbiological industry.

SUBSTANCE: biological preparations for controlling insect pests are obtained from entomonopathogenic nematodes and is characterized by that autoclaving of nutrient medium is carried out in a separate vessel, into which are then inoculated symbiotic bacteria and, after reproduction thereof, nutrient medium is transferred into another vessel simultaneously with inoculation of monoxenous nematode culture into the same second vessel. Production line is characterized by that bioreactor is constituted by two units: bioreactor for preliminary reproduction of symbiotic bacteria made in the form of polypropylene sac and bioreactor for growing nematode-bacteria complex made in the form of plastic trough with cover of perforated foil. Additionally are introduced: sterilization block for plastic troughs made using open flame and block for separating nematodes from inert vehicle made using common washing machine. Such structure of bioreactor allows one to create optimal conditions for vital activity of nematodes and their symbiotic bacteria.

EFFECT: increased stable specific yield of nematode biomass.

2 cl, 3 dwg, 2 tbl