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RU

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2 748 023

(13)

C1

(51)

IPC

C12N1/20(2006-01-01)

A01N63/12(2020-01-01)

A01N63/20(2020-01-01)

A01P7/04(2006-01-01)

(21) (22)

Application

2020137190, 2020-11-12

(24)

Start date

2020-11-12

(22)

Actual filing date

2020-11-12

(45)

Published

2021-05-19

(72)

Inventor

Danilov Leonid GrigorevichNashchekina Tatiana IurevnaZorina Elena Anatolevna

(73)

Holder

Federalnoe Gosudarstvennoe Biudzhetnoe Nauchnoe Uchrezhdenie Nauchno-Issledovatelskii Institut Zashchity Rastenii»

METHOD FOR OBTAINING MASS CULTURE OF SYMBIOTIC BACTERIA XENORHABDUS OF ENTOMOPATHOGENIC NEMATODES RHABDITIDA: STEINERNEMATIDAE Russian patent published in 2021 - IPC C12N1/20 A01N63/12 A01N63/20 A01P7/04 

Abstract RU 2748023 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. Disclosed is a method for obtaining a mass culture of symbiotic bacteria Xenorhabdus of entomopathogenic nematodes Steinernematidae, including the preparation of a pure culture of symbiotic bacteria and their cultivation on a nutrient medium used for the reproduction of nematode-bacterial complexes and containing egg powder - 6.4%; unrefined sunflower oil - 4.9%; soy flour - 11.6%; brewer's yeast - 0.7%; salt - 0.3%; water - 62.7%; foam rubber - 13.4%; moreover, the nutrient medium is additionally diluted with tap water in a ratio of 12%: 88%.

EFFECT: increased yield of culture of entomopathogenic nematodes Steinernematidae and their invasive activity against test insects in the presence of symbiont bacteria.

1 cl, 4 tbl, 1 ex

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RU 2 748 023 C1

Authors

Danilov Leonid Grigorevich

Nashchekina Tatiana Iurevna

Zorina Elena Anatolevna

Dates

2021-05-19—Published

2020-11-12—Filed

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