FIELD: biotechnology.
SUBSTANCE: invention relates to a method of stimulating invasive larvae of entomopathogenic nematodes of the genus Steinernema to the transition to the feeding stage, which consists in the fact that 50 g of live caterpillars of the large honeycomb moth Galleria mellonella are killed by placing them in a freezer at a temperature of -18°C for 1 hour, then placed in a homogenizer and a homogenate is made; Lurie agar is prepared according to the generally accepted method; 10% by weight of homogenate from caterpillars is added to Lurie agar; the mixture is cooled naturally, periodically shaking the flask for better mixing; the resulting medium is poured into Petri dishes; the mixture is autoclaved at 121°C for 25 minutes; a purified culture of bacteria of the genus Xenorhabdus is applied to the surface of the cooled mixture; two days later, larvae of non-feeding entomopathogenic nematodes of the genus Steinernema are introduced onto the surface of the mixture; their transition to the feeding stage is visually controlled by the appearance of muscular activity of the esophagus and ingestion of the medium with bacteria.
EFFECT: stimulating invasive larvae of entomopathogenic nematodes of the genus Steinernema to switch to the feeding stage, which makes it possible to use these entomopathogenic nematodes in the control of plant pests.
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