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2 444 195

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C2

(51)

IPC

A01N63/00(2006-01-01)

A01K67/33(2006-01-01)

(21) (22)

Application

2009145253/10, 2009-12-07

(24)

Start date

2009-12-07

(22)

Actual filing date

2009-12-07

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Published

2012-03-10

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Inventor

(73)

Holder

Danilov Leonid Grigor'Evich

METHOD OF PRODUCING ENTOMOPATHOGENIC NEMATODE-BASED BIOLOGICAL AGENTS Russian patent published in 2012 - IPC A01N63/00 A01K67/33 

Abstract RU 2444195 C2

FIELD: chemistry.

SUBSTANCE: invention relates to microbiological industry. The method involves filling bioreactors with a culture medium into which, after sterilisation and cooling, a liquid culture of symbiotic bacteria is inoculated and nematodes are inoculated therein after 48-hour exposure. The bioreactors re put into temperature-controlled chambers for development of a nematode-bacterial complex. The bioreactor is made in form of a separate polypropylene sack measuring 700×400 mm with 186 through holes with diameter of 0.5 mm on each side of the sack, at a distance of 190 mm from the top part of the sack and on an area of 1500 mm2 with height of 50 mm and 300 mm width of the sack.

EFFECT: method increases output of biomass and invasive activity of nematodes.

3 dwg, 2 tbl, 1 ex

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RU 2 444 195 C2

Authors

Dates

2012-03-10—Published

2009-12-07—Filed

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