FIELD: medicine, physiology.
SUBSTANCE: invention relates to creating cold-safety solution for freezing leukocytes and their retaining in physiologically active state after thawing. Cold-safety solution for freezing leukocytes in the ratio 1:1 at moderately low temperature and pH 7.0-7.4 contains hexamethylene-bis-tetraoxyethylurea and water for injection. Leukocytes are frozen to -20oC being solution contains additionally hydroxymethylethylpyridine succinate in the following ratio of components, %: hexamethylene-bis-tetraoxyethylurea, 26.0-30.0; hydroxymethylethylpyridine succinate, 0.1-0.2, and water for injection, up to 100 ml. Cold-safety solution in freezing leukocytes concentrate to -20oC provides the safety of high percent of physiologically active leukocyte cells.
EFFECT: valuable properties of solution.
3 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| CRYOPROTECTIVE SOLUTION FOR LEUKOCYTE FREEZING AT LOW TEMPERATURE | 2004 |
|
RU2290808C2 |
| CRYOPROTECTIVE SOLUTION FOR MODERATELY HYPOTHERMIC LEUKOCYTE PRESERVATION (-40°C) | 2009 |
|
RU2439877C2 |
| CRYOCONSERVING AGENT FOR LEUCOCYTES | 2006 |
|
RU2301524C1 |
| SOLUTION FOR LEUKOCYTE PRESERVATION AT NEAR-ZERO TEMPERATURE | 2006 |
|
RU2311027C1 |
| PROTECTIVE SOLUTION FOR PRESERVING LEUKOCYTE AT TEMPERATURE -10°C | 2004 |
|
RU2261595C1 |
| CRYOPROTECTIVE SOLUTION TO FREEZE LEUCOCYTES AT MODERATE-LOW TEMPERATURE | 2001 |
|
RU2184449C1 |
| SOLUTION FOR CELL SUSPENSIONS PRESERVATION | 2015 |
|
RU2621295C2 |
| FROST PROTECTION SOLUTION FOR NUCLEAR BLOOD CELL FREEZING | 2011 |
|
RU2464991C1 |
| COLD-SAFETY SOLUTION FOR FREEZING PLATELETS AT MODERATELY LOW TEMPERATURE | 2002 |
|
RU2230454C2 |
| COMBINED CRYOPROTECTIVE SOLUTION FOR THROMBOCYTE FREEZING | 2012 |
|
RU2477953C1 |
