FIELD: medicine, biotechnology.
SUBSTANCE: the present innovation deals with biotechnology of diagnostic preparations and could be applied at manufacturing medicinal immunobiological preparations indicated for detecting specific antibodies and antigens in biological substrates. One should carry out equine hyperimmunization with human immunoglobulin G or that of other animal purified with ion-exchange chromatography, serum should be separated, fermentolysis is carried out, then it is necessary to mix it with specific immunosorbent at constant mixing for 30 ± 5 min and air temperature being 22 ± 2 C and obtained F(ab)2 fragments of immunoglobulins against human IgG or those of another animal should be conjugated with PITC at 30 mg PITC/g protein at constant mixing for 19 ± 1 h and at 2-8 C. The method enables to achieve increased specificity and sensitivity of antispecies fluorescent conjugate for reaction of indirect immunofluorescence and increase the volume of immune serum obtained.
EFFECT: improved quality of target product.
4 ex, 2 tbl
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