FIELD: biotechnology.
SUBSTANCE: method involves preparing plants-donors of meristems for freezing, wherein plants-donors are toughened on solid medium supplemented with substances of cytokinic effect; separating apexes and treating with cryogenic protectors; cooling apexes in cryogenic ampoules and providing cryogenic keeping; performing post-cryogenic plant regeneration from cryogenically kept meristems. Method for cryogenic keeping of meristematic apexes isolated from in vitro toughened plants allows plants to be recovered from cryogenically kept meristems of red raspberry (Rubus idaeus L.), with callus forming stage being avoided.
EFFECT: increased efficiency and simplified method.
1 dwg, 2 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR CRYOPRESERVATION OF MERISTEMS ISOLATED OUT OF PLANTS OF COMMON GARDEN STRAWBERRY (FRAGARIA L | 2002 |
|
RU2220563C1 |
| METHOD FOR IN VITRO CRYOCONSERVATION OF MERISTEMS ISOLATED FROM STRAWBERRY (FRAGRARIA L) PLANTS | 2006 |
|
RU2302107C1 |
| METHOD OF CRYOPRESERVATION OF AXILLARY BUDS OF ASPEN PLANTS IN VITRO | 2013 |
|
RU2565803C2 |
| METHOD OF CRYOGENIC PRESERVATION OF GENETIC COLLECTION OF POTATO VARIETIES AND SPECIES | 0 |
|
SU1524479A1 |
| METHOD FOR PREPARATION OF IN VITRO PROPAGATED STRAWBERRY (FRAGRARIA L) PLANTS TO EX VITRO CULTIVATION | 2006 |
|
RU2302106C1 |
| METHOD OF GROWING CLOUDBERRIES (RUBUS CHAMAEMORUS LINNAEUS) | 2024 |
|
RU2824883C1 |
| METHOD FOR GROWING RUBUS ARCTICUS (RUBUS ARCTICUS L.) | 2023 |
|
RU2811144C1 |
| METHOD FOR CLONAL MICROPROPAGATION OF LANCEOLAR ASIAN BELL (CODONOPSIS LANCEOLATA (SIEBOLD. & ZUCC.) BENTH.& HOOK. FIL.) | 2018 |
|
RU2710535C1 |
| METHOD FOR PRODUCTION OF ARTIFICIAL SEEDS BASED ON MERISTEM OF SOLANUM TUBEROSUM L_ CULTURE | 2019 |
|
RU2723408C1 |
| METHOD OF PRESERVING ALIVE CELLS AND TISSUE OF PLANTS | 0 |
|
SU1144673A1 |
