FIELD: agriculture.
SUBSTANCE: method for growing Rubus arcticus can be used for accelerated biological production of healthy planting material. The method includes planting an explant on a nutrient medium, rooting in an in vitro culture to obtain regenerated plants, their microcuttings and rooting of microcuttings in an in vitro culture on a nutrient medium containing carbohydrates, subsequent adaptation of plants to non-sterile conditions ex vitro, while serving as a source of carbohydrates in the nutrient medium, fructose is used in the amount of 25.0 g/l of the nutrient medium, while at the stage of introduction into the culture in vitro, the fungicide Ordan is added to the sterilizing agents in a ratio of 1:5, at the stage of micropropagation itself, birch charcoal is added to the nutrient medium 8.0 g/l, colloidal cluster silver 1.0 ml/l and vitamin B12 in the amount of 0.3 ml/l, at the stage of rooting of microshoots in in vitro culture, 10.0 mg/l of succinic acid is added to the nutrient medium, adapt plants in vitro to soil conditions ex vitro, using coconut substrate with the addition of zeolite, which are taken in a ratio of 3:1.
EFFECT: increase in the reproduction rate of Rubus arcticus plants while reducing labor costs for obtaining seedlings.
1 cl, 5 tbl
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Authors
Dates
2024-01-11—Published
2023-10-18—Filed