METHOD OF GROWING AND MAINTAINING NON-DIFFERENTIATED HEMOPOIETIC STEM CELLS OR PRECURSORS CELLS (OPTIONS), METHOD OF PREPARING CONDITIONED STROMAL CELL MEDIUM, METHOD OF TRANSPLANTING NON-DIFFERENTIATED HEMOPOIETIC STEM CELLS OR PRECURSORS CELLS (OPTIONS) Russian patent published in 2005 - IPC

FIELD: biotechnology and pharmaceutical industry.

SUBSTANCE: title operations are accomplished by following way. Three-dimensional culture of stromal cells is cultured in piston flow bioreactor, in particular being introduced in fibrous matrix incorporated into substrate, which is placed in container constituting a part of bioreactor piston. Stromal cells are grown until density 5 x 10⁶ cell/cm³ substrate is attained, whereupon non-differentiated hemopoietic cells are either sowed directly into piston flow bioreactor or cultured in conditioned medium of stromal cells obtained by gathering medium from indicated flow bioreactor. Non-differentiated hemopoietic cells obtained by cultivation in presence of three-dimensional culture of stromal cells or their conditioned medium are transplanted to into recipient.

EFFECT: enabled growth of large amounts of stromal cells within a relatively small volume to provide longer maintenance of vital activity and reproduction of non-differentiated hemopoietic stem cells or precursor cells.

77 cl, 9 dwg, 3 tbl