FIELD: genetic engineering and medicine genetic.
SUBSTANCE: method for detection of gene resistance of subjects to infection by HIV1 is disclosed. Method includes DNA isolation, CCR5 gene PCR-amplification by using two primers complementary to two gene CCR5 sites. Further amplification products are restricted with endonuclease HincII (HindII), sizes of formed amplified fragments are determined, and on the base of obtained data deletion and single-nucleotide mutations are detected. Method of present invention makes it possible to simultaneously diagnose the presence of two mutation in human CCR5 gene, and (if individual has both mutation in heterozygote state) to distinguish cys- and trans-configurations.
EFFECT: method for diagnosis of congenital gene resistance to infection by HIV1.
2 dwg, 1 tbl, 2 ex
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| METHOD FOR DETERMINING CCR5 delta 32 ALLELE POLYMORPHISM | 2014 |
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| GENETIC CONSTRUCT FOR EXPRESSION OF mNG_CD4-CCR5 GENES, RECOMBINANT PLASMID rVSV_mNG_CD4-CCR5 AND RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS rVSV_mNG_CD4-CCR5 PROVIDING TARGETED VIROLYSIS OF CELLS DISPLAYING HIV-1 PROTEINS GP120/GP41 OF R5 TROPISM ON THEIR SURFACE | 2021 |
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| 02_AGRU09RU2204 TYPE 1 HUMAN IMMUNODEFICIENCY VIRUS STRAIN, SUBTYPE 02_AG USED FOR DIAGNOSING AND STUDYING EFFICACY OF THERAPEUTIC AND VACCINE PREPARATIONS | 2010 |
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| STRAIN 02-AG RU 09RU3124 OF HUMAN IMMUNODEFICIENCY VIRUS TYPE 1, RECOMBINANT SUBTYPE 02-AG USED FOR DIAGNOSING AND STUDYING EFFICACY OF THERAPEUTIC AND VACCINE PREPARATIONS | 2010 |
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