METHOD OF DETERMINING ALLELOPATHY ACTIVITY OF SOIL Russian patent published in 2005 - IPC

FIELD: biochemistry.

SUBSTANCE: method comprises using microscopic chlorella algae as a biological test, distributing the suspension of the cells of chlorella over the paper filter on the surface of the soil plate in the Petri caps, obtaining chlorophyll extract, determining optical density of the extract, and comparing it with the reference one. The 20-ml volume of the suspension of the chlorella cells are distributed inside the Petri caps. The caps are covered and set into a greenhouse. The caps are exposed to light during seven days, and then the filters are removed from the caps, dried at a temperature of 38-42°С, grinded, and extracted. The allelopathy activity of the soil is expressed in per cents of the optical density of the extract on the reference one, in which chlorella is grown on the filter, which is set onto four layers of moistened filtering paper or cotton.

EFFECT: reduced labor consumptions and enhanced reliability of determining.

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