FIELD: microbiology.
SUBSTANCE: method for enzymatic conversion of phytosterol compositions to androstenedion (androst-4-ene-3,17-lion, AD) and androstadienedion (androsta-1,4-diene-3,17-dion, ADD) includes heating of phytosterol composition in presence of one or more solubilizers up to 100-130°C to form paste-like solution. Solubilizers are selected from polypropylene glycol, silicone or vegetable oil. Prepared paste-like mass is charged into bioreactor containing microorganism Mycobacterium MB 3683 and inorganic salt medium followed byfermentation. Method of present invention makes it possible to sufficiently increase of phytosterol composition concentration (30 g or more for 1 l of broth) and to increase produced product yield up to 80-90 %.
EFFECT: method of improved efficiency and yield.
6 cl, 1 dwg, 2 tbl, 5 ex
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