METHOD FOR PRODUCING BACTERIOCHLOROPHYLL A Russian patent published in 2018 - IPC C12P17/18 C12N1/20 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. Method of periodic fed-batch fermentation for production of bacteriochlorophyll a (Bchla) is proposed. Method comprises cultivation cells of a strain DSM 1374 Rhodovulum sulfidophilum in a growth medium containing citric acid, CaCl₂*2H₂O, MgSO₄*7H₂O, citrate FeNH₄, succinic acid, disodium succinate*6H₂O, Na₂SO₄, K₂CO₃, yeast extract, NH₄Cl, KH₂PO₄, K₂HPO₄*3H₂O, NaCl, NaOH, KOH, anti-foam agent and microelements. In the fermentation process, aqueous solution of succinic acid and disodium succinate, aqueous solution NH₄Cl, KH₂PO_4, K₂HPO₄*3H₂O from external reservoirs connected to the fermenter vessel, and keeping the oxygen level at or lower than 10 %. After fermentation completion, Bchla is separated from isolated cells.

EFFECT: invention provides increase in the yield of the desired product.

5 cl, 4 ex