FIELD: biochemistry, gene engineering, in particular amidation of hormones and other peptides used in medicine and agriculture.
SUBSTANCE: peptide sequence obtained in process of triptic digesting of peptidylglycine alpha-amidating monooxygenase (PAM) from fat thyroid encephaloid carcinoma is determined. On the base of obtained data oligonucleotide sequences are synthesized and used for isolation of full-sized PAM encoding DNA. Expression of obtained DNA fragments in heterogeneous systems makes it possible to obtain recombinant PAM useful in amidation reaction of peptide substrate with C-terminated glycine providing the same effect that for native enzyme.
EFFECT: effective method for production of amidated peptides.
12 dwg, 2 tbl, 9 ex
| Title |
Year |
Author |
Number |
| $$$-AMIDATING MONOOXYGENASE (VARIANTS) AND A METHOD OF PREPARING $$$-AMIDATED FORM OF REGULATORY POLYPEPTIDE |
1988 |
- Dzhejms P.Dzhilligen[Us]
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- Artur Kh.Bertel'Zen[Us]
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|
RU2080385C1 |
| METHOD OF PEPTIDYLGLYCINE @@@-AMIDATING MONOOXYGENASE PURIFICATION AND A METHOD OF @@@-AMIDATED PEPTIDE PREPARATION |
0 |
- Dzhejmz P.Dzhilligan
- Berri N.Dzhounz
|
SU1802814A3 |
| STRAIN ESCHERICHIA COLI JM 105 TRANSFORMED WITH PLASMID PAE12 - PRODUCER OF ALPHA-AMIDATING ENZYME, RECOMBINANT PLASMID PAE12, MURINE CELLULAR LINE C127 TRANSFORMED WITH PLASMID PDBPV-MMTNEO α - PRODUCER OF ALPHA-AMIDATING ENZYME, RECOMBINANT PLASMID PDBPV-MMTNEO α |
1990 |
- Artur Kh.Betelsen
- Nozer M.Mekhta
- Gehri Ehdzhajd B'Judraj
|
RU2129606C1 |
| PRODUCTION OF PEPTIES AND PROTEINS BY MEANS OF ACCUMULATION IN ENDOPLASMIC RETICULUM OF PLANTS |
2002 |
- Ljudevid Mugika Marija Dolores
- Torrent Ketglas Margarita
- Lasser-Ramassami Sabina
|
RU2381275C2 |
| POLYPEPTIDE ELICITING URATE OXIDASE ACTIVITY, DNA FRAGMENT DETERMINING EXPRESSION OF POLYPEPTIDE ELICITING URATE OXIDASE ACTIVITY (VARIANTS), EXPRESSION VECTOR CONTAINING DNA FRAGMENT DETERMINING EXPRESSION OF POLYPEPTIDE ELICITING URATE OXIDASE ACTIVITY (VARIANTS), STRAIN EXPRESSING POLYPEPTIDE ELICITING URATE OXIDASE ACTIVITY (VARIANTS), METHOD OF PRODUCING POLYPEPTIDE ELICITING URATE OXIDASE ACTIVITY |
1991 |
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- Murad Kagad[Fr]
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- Jokhannes Lupker[Nl]
- Paskal' Leplatua[Fr]
- Mark Salom[Fr]
- Patrik Loran[Fr]
|
RU2105812C1 |
| NOVEL PROTEINS AND METHODS OF THEIR PREPARING |
1996 |
- Goto Masaaki
- Tsuda Ehjsuke
- Motizuki Siniti
- Jano Kazuki
- Kobajasi Fumie
- Sima Nobujuki
- Jasuda Khisataka
- Nakagava Nobuaki
- Morinaga Tomonori
- Ueda Masatsugu
- Khigasio Kandzi
|
RU2194714C2 |
| RECOMBINANT PREPARATION OF PEPTIDES |
2010 |
- Khjummerikh Daniehl'
- Libmann Burgard
- Fer Markus
- Shval'B Karsten
- Brjuzer Khajke
|
RU2548815C2 |
| PROTEIN ABLE TO INHIBIT OSTEOCLASTOGENESIS (OCIF) (VARIANTS), CDNA, METHOD FOR PREPARING PROTEIN, METHOD FOR TREATMENT OF OSTEOPOROSIS, METHOD FOR TREATMENT AND/OR IMPROVEMENT OF BONE MASS RECOVERY, METHOD FOR TREATMENT OF INJURE ASSOCIATED WITH OSSEOUS METABOLISM, TRANSFORMED STRAIN ESCHERICHIA COLI PBK/01F10 AND PHARMACEUTICAL COMPOSITION |
1996 |
- Goto Masaaki
- Tsuda Ehjsuke
- Motizuki Siniti
- Jano Kazuki
- Kobajasi Fumie
- Sima Nobujuki
- Jasuda Khisataka
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- Morinaga Tomonori
- Ueda Masatsugu
- Khigasio Kandzi
|
RU2238948C2 |
| METHOD OF PRODUCING RECOMBINANT BETA-N-ACETYLGLUCOSAMINIDASE StrH FROM STREPTOCOCCUS PNEUMONIAE |
2018 |
- Ershov Aleksandr Viktorovich
- Ershova Olga Anatolevna
- Leonov Vyacheslav Sergeevich
|
RU2693660C1 |
| IMMUNOGLOBULIN POLYPEPTIDE, ITS FRAGMENT, COMPOSITION, STRAIN OF HYBRID CULTURED CELLS, METHOD OF POLYPEPTIDE PREPARING |
1992 |
- Ramakrishnan Vanita
- Ehskobedo Marija A.
- Fretto Lehrri Dzh.
|
RU2194760C2 |
