FIELD: biotechnologies.
SUBSTANCE: plants are transformed with application of nucleic-acid constructs, which contain the first nucleotide sequence that codes γ-sein, or its fragment, which is able to direct and retain protein in endoplasmic reticulum of plant cell, the second sequence of nucleic acid, which codes aminoacid sequence, which is specifically split by ferment or chemical compounds, and the third sequence of nucleic acid, which codes target peptide or protein.
EFFECT: transformation of plant by such constructs makes it possible to produce fused proteins accumulated in endoplasmic reticulum of cells in the form of protein bodies, from which target proteins may be extracted, in particular calcitonin.
47 cl, 19 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| PROTEIN PRODUCTION | 2005 |
|
RU2429243C2 |
| PROTEIN RECOVERY AND PURIFICATION | 2005 |
|
RU2420585C2 |
| PRODUCTION OF BIOLOGICALLY ACTIVE PROTEINS | 2007 |
|
RU2441911C2 |
| PESTICIDE COMPOSITIONS AND RELATED METHODS | 2013 |
|
RU2662672C2 |
| VEGETABLE PEPTIDE GAMMA-ZEIN FOR DELIVERY OF BIOMOLECULES TO VEGETABLE CELLS | 2011 |
|
RU2563805C2 |
| WHEAT PLANTS WITH HIGHER RESISTANCE TO IMIDAZOLINONE HERBICIDES | 2002 |
|
RU2337532C2 |
| SYNTHETIC TRANSIT CHLOROPLAST PEPTIDES FROM BRASSICA | 2013 |
|
RU2636035C2 |
| TRANSGENIC PLANTS RESISTANT TO BACTERIA | 2012 |
|
RU2640246C2 |
| ISOLATED NUCLEIC ACID, ENCODING OPERATIVELY FUSED INTRAMOLECULAR DIMER OF KillerRed PROTEIN, EXPRESSION CASSETTE, CELL, WHICH PRODUCES CHIMERIC PROTEIN AND CONTAINS EXPRESSION CASSETTE, ISOLATED CHIMERIC PROTEIN | 2011 |
|
RU2491342C1 |
| ISOLATED KillerRed PROTEIN-CODING NUCLEIC ACID (VERSIONS), ISOLATED PROTEIN (VERSIONS), EXPRESSION CASSETTE, CELL CONTAINING EXPRESSION CASSETTE | 2011 |
|
RU2458129C1 |
