$$$-AMIDATING MONOOXYGENASE (VARIANTS) AND A METHOD OF PREPARING $$$-AMIDATED FORM OF REGULATORY POLYPEPTIDE Russian patent published in 1997 - IPC

FIELD: biotechnology, enzymes. SUBSTANCE: $$$-amidating monooxygenases were isolated and purified from tissue and cellular lines of cerebriform thyroid gland carcinoma that are able to catalyze peptide substances transformation with C-terminal glycine residue involving natural L-forms to the corresponding peptidylamide. To obtain active $$$-amidated forms of regulatory peptides (calcitonine, growth hormone factor release and others) the natural or recombinant precursor with amino acid sequence of amidated regulatory peptide and supplementary glycine residue by C-end is treated with the prepared enzyme. EFFECT: improved of enzymes preparing. 12 cl, 22 dwg, 2 tbl