FIELD: biotechnologies.
SUBSTANCE: invention relates to the biotechnology. Disclosed is an industrial method of producing a biologically active preparation of recombinant exo-beta-N-acetylglucosaminidase StrH (β-N-GlcNAcase StrH) produced in accordance with GMP rules. Method includes consecutive stages of metal chelate chromatography for sorption of target protein with hexahistidine tag; gel filtration in breakthrough mode to purify the target protein from contaminating impurities of cell proteins which are coelaxing with the target protein at all steps; anion-exchange chromatography on sorbent, optimized for capture and purification of proteins with high molecular weight; hydrophobic chromatography, where the contaminant impurities of hydrophobic proteins and bacterial endotoxins are purified.
EFFECT: method is suitable for use at the remodeling step in preparing the therapeutically functional preparations of glycoproteins with given properties, as well as for developing tools for high-sensitivity analysis of glycan chains of glycoproteins of plant tissues, animals and eukaryotic microorganisms.
9 cl, 8 dwg, 7 tbl, 8 ex
| Title |
Year |
Author |
Number |
| METHOD OF PRODUCING RECOMBINANT Β-1,4-GALACTOSIDASE BGAA FROM STREPTOCOCCUS PNEUMONIAE |
2019 |
- Ershov Aleksandr Viktorovich
- Ershova Olga Anatolevna
- Leonov Vyacheslav Sergeevich
|
RU2698460C1 |
| METHOD OF PRODUCING RECOMBINANT NEURAMINIDASE NanH FROM CLOSTRIDIUM PERERINGENS |
2018 |
- Ershov Aleksandr Viktorovich
- Ershova Olga Anatolevna
- Leonov Vyacheslav Sergeevich
|
RU2698774C1 |
| METHOD FOR PRODUCING SERRATIA MARCESCENS RECOMBINANT ENDONUCLEASE |
2021 |
- Ershov Aleksandr Viktorovich
- Ershova Olga Anatolevna
- Anisimov Roman Lvovich
- Katorkin Sergej Aleksandrovich
- Bondar Nikolaj Igorevich
|
RU2795623C2 |
| METHOD OF PRODUCING A RECOMBINANT NUCLEASE CAS ESSENTIALLY FREE OF BACTERIAL ENDOTOXINS, THE PREPARATION OBTAINED BY THIS METHOD AND CONTAINING A KIT FOR USE IN A CRISPR/Cas SYSTEM |
2019 |
- Akimkin Vasilij Gennadevich
- Tyumentsev Aleksandr Igorevich
- Tyumentseva Marina Alekseevna
- Shagin Dmitrij Alekseevich
|
RU2707542C1 |
| METHOD OF INDUSTRIAL PURIFICATION OF ROMIPLOSTIM |
2021 |
- Ershov Aleksandr Viktorovich
- Ershova Olga Anatolevna
- Anisimov Roman Lvovich
- Katorkin Sergej Aleksandrovich
|
RU2804622C2 |
| METHOD OF PRODUCING HUMAN RECOMBINANT ANTI-TUMOUR PROTEIN DR5-B |
2018 |
- Gasparyan Marine Eduardovna
- Yagolovich Anna Valerevna
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2687435C1 |
| RECOMBINANT PLASMID DNA OF PQE-30_P36GP12_GP57, PROVIDING SYNTHESIS OF RECOMBINANT PROTEIN P36GP12 IN CELLS OF ESCHERICHIA COLI, A STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF RECOMBINANT PROTEIN P36GP12, RECOMBINANT PROTEIN P36GP12, CAPABLE TO BIND LIPOPOLYSACCHARIDES ESCHERICHIA COLI |
2019 |
- Bokovaya Olga Vasilevna
- Bajkov Ivan Konstantinovich
- Morozova Vera Vitalevna
- Kozlova Yuliya Nikolaevna
- Tikunova Nina Viktorovna
|
RU2707922C1 |
| METHOD FOR PRODUCING A SUBSTANCE OF THE RECOMBINANT L-ASPARAGINASE FROM ERWINIA CAROTOVORA |
2010 |
- Karasev Viktor Semenovich
- Bochkova Ol'Ga Petrovna
- Chugunov Aleksandr Mikhajlovich
- Melik-Nubarov Nikolaj Sergeevich
- Grozdova Irina Dmitrievna
- Chernovskaja Tat'Jana Veniaminovna
- Denisov Lev Aleksandrovich
- Rudenko Elena Georgievna
- Morozova Elena Leonidovna
- Bogush Vladimir Grigor'Evich
- Sidoruk Konstantin Vasil'Evich
- Koltun Igor' Olegovich
- Skatova Galina Evgen'Evna
- Abakumova Ol'Ga Jur'Evna
- Podobed Ol'Ga Vladimirovna
- Sokolov Nikolaj Nikolaevich
|
RU2441914C1 |
| PLASMID FOR EXPRESSION IN CELLS OF BACTERIUM BELONGING TO Escherichia CLASS, NON-ACTIVE PREDECESSOR OF DNase I OF HUMAN OR ITS MUTEINS; BACTERIUM BELONGING TO Escherichia CLASS, - PRODUCER OF NON-ACTIVE PREDECESSOR OF RECOMBINANT DNase I OF HUMAN OR ITS MUTEIN; PREDECESSOR OF RECOMBINANT DNase I OF HUMAN OR ITS MUTEIN; METHOD FOR OBTAINING RECOMBINANT DNase I OF HUMAN OR ITS MUTEIN; METHOD FOR OBTAINING CONJUGATES OF POLYETHYLENE GLYCOL AND RECOMBINANT MUTEIN OF HUMAN DNase I, FERMENTATIVE ACTIVE CONJUGATE OF MUTEIN OF RECOMBINANT DNase I OF HUMAN |
2011 |
- Orlova Nadezhda Aleksandrovna
- Vorob'Ev Ivan Ivanovich
|
RU2502803C2 |
| PLASMID FOR EXPRESSION IN BACTERIA CELLS OF GENUS Escherichia OF INACTIVE PRECURSOR OF MUTEIN [C112S] OF HUMAN ENTEROKINASE LIGHT CHAIN, BACTERIA BELONGING TO GENUS Escherichia, - PRODUCER OF PRECURSOR OF RECOMBINANT MUTEIN [C112S] OF HUMAN ENTEROKINASE LIGHT CHAIN, PRECURSOR OF RECOMBINANT MUTEIN [C112S] OF HUMAN ENTEROKINASE LIGHT CHAIN, METHOD OF PRODUCTION OF RECOMBINANT MUTEIN [C112S] OF HUMAN ENTEROKINASE LIGHT CHAIN, RECOMBINANT MUTEIN [C112S] OF HUMAN ENTEROKINASE LIGHT CHAIN |
2011 |
- Orlova Nadezhda Aleksandrovna
- Vorob'Ev Ivan Ivanovich
- Matskevich Viktor Aleksandrovich
- Zvjagin Ivan Vladimirovich
|
RU2495934C2 |
