FIELD: microbiology, immunology, veterinary.
SUBSTANCE: vaccine strain Brucella abortus 104 biovar.1 is cultivated and microbial cells are washed. Then precipitate is resuspended with alkali, preferably 0.5 N NaOH solution, under continuous agitation to produce finished microbial cell concentration of 1x1011 cells in 1 ml. Product is hydrolyzed at 55-57°C under continuous agitation for 10-12 min followed by cooling at 4-6°C for 30 min or more. Suspension is neutralized with 2 N acetic acid and antigen is precipitated with ethanoly preferably in ratio brucellosis antigen/ethanol of 1:10. Method of present invention makes it possible to increase brucellosis antigen yield up to 30 % (calculated as dry microbial mass).
EFFECT: simplified, accelerated and effective method for brucellosis antigen production.
3 cl, 2 tbl, 1 ex
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