FIELD: biotechnology, production of polygalacturonase enzyme preparation useful in wine production and hydrolysis industry.
SUBSTANCE: claimed method includes the first concentration of cultural liquid or solution of crude enzyme preparation and solution demineralization. Second enzyme concentration is carried out directly in column by solution passing through column filled with hydroxyapatite with granulation of 100-250 mum at 3-5°C. Columns are washed with 10 mM phosphate buffer with pH 6.0-7.0 followed by fraction elution by phosphate buffer gradient. Active fraction is subjected to gel chromatography and enzyme preparation is lyophilized.
EFFECT: method with increased yield, purification ratio, productivity and decreased dilution of active fraction.
2 cl, 1 tbl, 3 ex
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