FIELD: microbiology, optics.
SUBSTANCE: invention relates to investigations of materials by assay of their physical or chemical properties using optical devices and to systems wherein material is excited by optical agents resulting to it luminescence. Invention proposes a test carrier as a centrifugal tube. Carrier is separated for upper and bottom cavities by partition. Volume of lower cavity is 0.1 of tube volume. A hole is made in partition near a wall. The constructive decision of partition provides efflux of sample from lower cavity with minimal overcoming the combined forces of wetting and surface tension. Also, invention proposes methods/variants for rapid measurement of absolute concentration of microorganisms in biosubstrate by their photoluminescence. Methods involve using fluorescent or phosphorescent measuring device and above said test carrier. Methods provides increasing rate and precision of assay, to use serial measuring devices and to carry out measurement of the concentration of particles in substrate with another specific gravity value as compared with that of liquid in substrate. Invention can be used in food and biotechnological industry for determination of absolute concentration of microorganisms in different substrates.
EFFECT: improved method for assay, valuable properties of carrier.
2 tbl, 1 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| LASER-LUMINESCENT SENSOR FOR CONCENTRATION MEASUREMENT WITH CONTACT MAKER OF BUNDLE FOR MINUTE MEASURING OF TERRAIN CLEARANCE CONCENTRATION OF LUMINESCENTS AND METHOD OF ITS USE | 2006 |
|
RU2347211C2 |
| LASER-LUMINESCENT CONCENTRATOR, METHOD OF ITS APPLICATION AND METHOD OF PRODUCING OPTIC WAVEGUIDE TIP (VERSIONS) | 2005 |
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| KNAPSACK LASER-SPECTROMETER-COMPUTER (LASCOM) CONCENTRATION METRE | 2010 |
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| LUMINESCENCE METHOD AND DEVICE FOR DIAGNOSING AND/OR ESTIMATING STATE QUALITY OF BIOLOGICAL OBJECT | 2004 |
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RU2254372C1 |
| DIFFERENTIAL DIAGNOSIS METHOD FOR MICROBES AND AMINO ACIDS | 2007 |
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| METHOD OF DETERMINING MICROBE DEATH RATE | 2007 |
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RU2396352C2 |
| METHOD FOR DETECTING AND IDENTIFYING MICROORGANISM CELLS AND VIRUSES | 2001 |
|
RU2197732C1 |
| METHOD OF MULTYANALITE IMMUNOASSAY WITH USE OF MICROPARTICLES | 2007 |
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RU2339953C1 |
| METHOD OF DETERMINATION OF ORAL CAVITY HYGIENE USING SPECTROLUX METHOD | 2006 |
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RU2351274C2 |
