METHOD OF MULTYANALITE IMMUNOASSAY WITH USE OF MICROPARTICLES Russian patent published in 2008 - IPC G01N33/58 G01N33/53 

FIELD: medicine.

SUBSTANCE: on surface of porous membrane apply the reactionary admixture containing analyte, the first binding molecules bound to detecting substance and specific to analyte, the investigated sample and the particles, not capable to pass through the pores of a membrane covered with the second binding molecules, also specific to analyte, incubate an admixture for formation of a biospecific complex, wash an admixture from not bound reagents and register in a regimen of the time permission phosphorescence signals in spectral ranges of the detecting substances corresponding to a constant of time of attenuation of these substances. Determine the required analyte on a parity of measured phosphorescence signals, thus use on two kinds of the first and second binding molecules, each kind of the first binding molecule is bound to two detecting is long luminescing substances, for example chelate of europium and platinaporphyrine which parity of concentration in each first binding molecule is chosen in advance and corresponds to defined analyte.

EFFECT: method allows finding out and quantitatively to detect low concentration biological analytes in assays at carrying out of researches on solid surface.

5 cl, 5 tbl, 3 dwg