DETERMINATION OF HYDROCOLE Daphnia magna STRAUS SENSIBILITY TO TOXIC EFFECT OF WATER-SOLUBLE CHEMICALS Russian patent published in 2005 - IPC

FIELD: toxicology, in particular determination of water flea sensibility to toxic effect of water-soluble chemicals.

SUBSTANCE: claimed method includes detection of water flea death time (min) caused by water-soluble chemicals, wherein concentration (C, mol/l) of chemical under consideration fluctuates according to logarithmic scale with interval of 0.1. Plot of Y versus X is made, wherein Y-axis represents average death time with scale of 1 point = 1 min; X-axis represents reverse concentration (1/C) of chemical under consideration; and scale is proportional to log increasing by 0.1. Water flea sensibility to toxic effect (tgα) is calculated according to equation: tgα = T_L(min):1/K_L = T_L(min)xK_l (I), wherein α is inclination of straight line to X-axis; T_L(min) is death time (min) being determined according to point of hypothetical crosspoint of straight line with Y-axis; K_L is lethality constant (mol/l) defined as chemical concentration wherein water flea death time is equal to 2T_L(min).

EFFECT: Method allowing evaluation of toxic effect evolution dynamics and comparison of toxic effect of water-soluble chemicals in equal concentration ranges.

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