METHOD FOR DETECTION OF MICROORGANISMS USING ISOTHERMIC AMPLIFICATION REACTION - RAMIL (REVERTASE AMPLIFICATION ILLATION) OR RNASE H-DEPENDENT AMPLIFICATION ILLATION Russian patent published in 2005 - IPC

Abstract RU 2258740 C2

FIELD: biochemistry, molecular biology.

SUBSTANCE: invention proposes method of isothermic amplification reaction that involves incubation in buffer system of a single-stranded analyzed nucleic acid sample with reverse transcriptase M-MuLV or AMV with Klenow's fragment (3' - 5' exo-) or without the latter, deoxyribonucleoside triphosphates and two pairs of specific ribooligonucleotide primers wherein sequence of the first pair of primers is identical with 5'-terminal sequence of sense chain of analyzed nucleic acid and sequence of the second pair of primers is complementary with sense chain. Primers of each pair differ by the presence of one of additional nucleotides by 3'-end. Applying of invention provides reducing cost of detection of microorganisms. Invention can be used in carrying out the molecular-genetic diagnosis.

EFFECT: improved method for detection of microorganisms.

2 cl, 3 dwg

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RU 2 258 740 C2

Authors

Vafin R.R.

Zajnullin L.I.

Akhmadeev R.M.

Ravilov R.Kh.

Vafin R.R.

Gabbasov I.M.

Dates

2005-08-20—Published

2003-08-21—Filed