FIELD: biochemistry, molecular biology.
SUBSTANCE: invention proposes variants of a method for detecting the sequence in DNA- or RNA-"target" that is based on carrying out the primer splicing reaction under conditions providing the definite limit of the product size (isometric primer splicing). Indicated conditions involve using at stage for primer splicing hybridized preliminary with the corresponding sequence site - a "target", the reaction mixture, wherein at least one of types of dNTP (deoxynucleoside triphosphate) is absent or replaced with the corresponding terminating substrate. The usage of proposed method provides significant enhancing sensitivity of analysis and to reducing time for its carrying out. Invention can be used in diagnostic tests and analytical systems for any designations suggesting detection of specific nucleic acid sequence in the sample.
EFFECT: improved detecting method.
44 cl, 2 dwg, 2 tbl, 1 ex
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