FIELD: biotechnology, microbiology, amino acids.
SUBSTANCE: invention proposes the strain Enterobacter agglomerans/FERM BP-7207 that is able to metabolize the carbon source at pH value when L-glutamic acid precipitates in liquid cultural medium containing L-glutamic acid in the saturation concentration and the carbon source. Also, the strain is able to accumulate L-glutamic acid in the amount exceeding the amount that corresponds to its saturation concentration in liquid cultural medium at this pH value. Also, invention relates to a method for preparing L-glutamic acid by fermentation that involves culturing this microorganism in liquid cultural medium wherein pH value is brought about to the value when L-glutamic acid precipitates to prepare and accumulate L-glutamic acid and to precipitate L-glutamic acid in this cultural medium. The proposed microorganism is prepared by addition of a sample comprising microorganisms to acid cultural medium containing L-glutamic acid in the saturation concentration and the carbon source followed by removal of strain that is able to metabolize this carbon source. Invention provides preparing L-glutamic acid with the high degree of effectiveness.
EFFECT: improved preparing method, valuable properties of strain.
20 cl, 9 dwg, 3 tbl, 8 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR PRODUCTION OF L-GLUTAMIC ACID | 2002 |
|
RU2282662C2 |
METHOD FOR PREPARING L-GLUTAMIC ACID | 2002 |
|
RU2288271C2 |
METHOD OF L-GLUTAMIC ACID PRODUCING | 1999 |
|
RU2188236C2 |
ORGANIC NITROGEN-CONTAINING COMPOSITION AND FERTILIZER COMPRISING THEREOF | 2002 |
|
RU2291139C2 |
METHOD OF L-GLUTAMIC ACID PRODUCING | 1999 |
|
RU2194076C2 |
METHOD FOR PRODUCTION OF L-AMINO ACID | 2003 |
|
RU2307165C2 |
METHOD OF OBTAINING L-AMINO ACID OF GLUTAMATE FAMILY, OR L-BALINE WITH APPLICATION OF BACTERIUM BELONGING TO GENUS Escherichia | 2009 |
|
RU2418064C2 |
METHOD FOR PREPARING L-AMINO ACIDS USING MICROORGANISM BELONGING TO GENUS ESCHERICHIA | 2005 |
|
RU2311454C2 |
METHOD FOR PRODUCTION OF L-AMINO ACIDS BELONGING TO GENUS ESCHERICHA | 2005 |
|
RU2304615C2 |
METHOD FOR OBTAINING L-THREONINE USING BACTERIUM RELATING TO Escherichia, IN WHICH rcsA GENE IS INACTIVATED | 2006 |
|
RU2359029C2 |
Authors
Dates
2005-09-27—Published
2000-08-18—Filed