METHOD FOR PREPARING L-GLUTAMIC ACID Russian patent published in 2006 - IPC C12P13/14 C12N1/21 C12R1/00 

FIELD: biotechnology, amino acids, microbiology.

SUBSTANCE: invention relates to a method for preparing L-glutamic acid by fermentation including culturing microorganisms possessing the ability for producing L-glutamic acid in medium with pH 5.0 or less wherein the total content of organic acid that inhibits the microorganism growth at this pH corresponds to the amount wherein the growth of microorganisms is not in inhibited. Also, L-glutamic acid is prepared by fermentation that includes culturing microorganism possessing the ability for producing L-glutamic acid at the first pH value wherein organic acid in medium doesn't inhibit the growth of microorganism followed by culturing microorganism at the second pH value suitable for production of L-glutamic acid by microorganism and at value lower as compared with the first value of pH. Microorganism can metabolize the carbon source in liquid medium containing L-glutamic acid at the saturation concentration and carbon source at pH 5 or less and able to accumulate L-glutamic acid in the amount exceeding the saturation concentration of L-glutamic acid. The claimed invention provides preparing L-glutamic acid with the high effectiveness degree.

EFFECT: improved preparing method.

10 cl, 13 dwg, 2 tbl, 1 ex